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Microcentrifuge

Microcentrifuge

The Microcentrifuge is an essential tool for any medical laboratory, offering high-speed centrifugation to separate micro-sized particles and molecules. Its compact design and powerful motor make it ideal for quick and efficient sample processing, with the ability to reach speeds of up to 15,000 RPM. The digital display and easy-to-use control panel allow for precise time and speed adjustments, ensuring reliable and consistent results. With a variety of rotor options available, this microcentrifuge is versatile enough to accommodate a wide range of sample sizes and types. Whether for DNA isolation, protein purification, or cell culture work, this microcentrifuge is a reliable and indispensable tool for any medical laboratory.
  • Applications

    • DNA and RNA Extraction: Microcentrifuges are essential for isolating nucleic acids such as DNA and RNA from biological samples. After lysis and centrifugation steps, the nucleic acids are separated from cellular debris and other components, facilitating downstream applications like PCR, sequencing, and cloning.
    • Protein Precipitation and Purification: Microcentrifuges are used in protein biochemistry for precipitating proteins from cell lysates or culture supernatants. Following centrifugation, the protein pellet can be resuspended and further purified for biochemical assays or structural studies.
    • Cell Pelleting: In cell biology, microcentrifuges are employed to pellet cells after various treatments such as washing steps, harvesting cells from culture, or separating cellular organelles. This allows for the collection of intact cells or organelles for further experimentation.
    • Immunoprecipitation (IP) and Co-Immunoprecipitation (Co-IP): Microcentrifuges play a crucial role in immunoprecipitation experiments where specific proteins are isolated from complex biological mixtures using antibodies. Following the binding of the target protein to the antibody, centrifugation is used to collect the immune complexes for analysis.
    • Enzyme Kinetics Studies: Microcentrifuges are used in enzymology for studying enzyme kinetics. By centrifuging reaction mixtures at various time points, researchers can separate enzyme and substrate, enabling the quantification of reaction products to determine enzyme activity and kinetics.
    • Sample Concentration and Desalting: Microcentrifuges equipped with ultrafiltration devices or spin columns are utilized for concentrating dilute samples or removing unwanted salts and small molecules. This is particularly useful in molecular biology and biochemistry for concentrating proteins, DNA, or RNA prior to analysis.
    • Buffer Exchange: Microcentrifuges are employed for rapid buffer exchange in molecular biology applications. By centrifuging samples through appropriate membranes or spin columns, researchers can exchange buffers efficiently, facilitating downstream applications such as protein refolding or changing reaction conditions.
    • Quick Spin-downs: Microcentrifuges are commonly used for quick spin-downs of reagents, PCR tubes, and microcentrifuge tubes to collect liquid at the bottom of the tube. This is essential for ensuring homogeneity of reaction mixtures, removing bubbles, and preventing sample loss during pipetting.
    • Sample Storage: Microcentrifuges are used for preparing samples for long-term storage. After centrifugation to remove particulates, samples can be aliquoted and stored at low temperatures, preserving their integrity for future analysis.
    • Microbial Culture Analysis: Microcentrifuges are utilized in microbiology for pelleting microbial cells from liquid cultures. This is essential for various applications including determining cell density, analyzing cell morphology, and extracting intracellular components.

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