Laser Scanning Microscopes

• Biological Research: LSMs are extensively used in biological research for imaging various biological specimens with high resolution. They are particularly useful for observing cellular structures, subcellular organelles, and dynamic processes such as cellular migration, mitosis, and apoptosis. LSMs enable researchers to study living cells and tissues in real-time without causing damage, making them invaluable for understanding biological processes at the microscopic level.
• Neuroscience: LSMs play a crucial role in neuroscience by enabling researchers to visualize neuronal structures and activities with exceptional clarity. They are used for studying the morphology of neurons, synaptic connections, and neuronal dynamics. LSMs equipped with calcium indicators can also monitor neuronal activity and study complex neural circuits in living organisms, providing insights into brain function and neurological disorders.
• Materials Science: LSMs are employed in materials science for characterizing the microstructure and surface morphology of materials at the nanoscale. They enable researchers to study the composition, defects, and interfaces of various materials, including metals, ceramics, polymers, and composites. LSMs are particularly useful for investigating thin films, nanomaterials, and nanostructures, facilitating the development of advanced materials with tailored properties for diverse applications.
• Medicine and Biomedical Imaging: LSMs are used in medicine for biomedical imaging applications such as studying tissues, organs, and pathological conditions at the cellular level. They are employed in disciplines like histology, pathology, and medical diagnostics to visualize cellular abnormalities, tissue architecture, and disease progression. LSMs can aid in early disease detection, monitoring treatment responses, and advancing our understanding of complex diseases like cancer, cardiovascular disorders, and neurodegenerative diseases.
• Confocal Microscopy: Confocal LSMs, a subtype of laser scanning microscopes, are widely utilized in various fields for three-dimensional imaging and optical sectioning. They eliminate out-of-focus light, resulting in sharper images with improved contrast and resolution. Confocal LSMs find applications in cell biology, developmental biology, neuroscience, and materials science for studying three-dimensional structures, spatial distributions, and interactions within biological specimens and materials.
• Live Cell Imaging: LSMs equipped with live-cell imaging capabilities are used for studying dynamic cellular processes in real-time. They enable researchers to observe cellular events such as cell division, migration, signaling, and gene expression with high temporal and spatial resolution. Live-cell imaging with LSMs is essential for understanding fundamental biological processes, elucidating disease mechanisms, and developing novel therapeutic interventions.
• Environmental Science: LSMs are utilized in environmental science for studying microorganisms, pollutants, and geological samples. They enable researchers to analyze microbial communities, study the effects of environmental factors on microbial growth and activity, and investigate the distribution of pollutants in environmental samples. LSMs are also employed in geology for studying rock formations, mineral composition, and sedimentary structures at the microscopic level, contributing to our understanding of Earth's history and processes.

• TruSpectral technology achieves up to 3X more light transmission vs. traditional spectral detection technology by implementing the volume phase holographic (VPH) diffraction grating.
• Offers independently adjustable channels to optimize signal detection for each individual fluorophore. Variable barrier filter mode provides simultaneous four-channel image acquisition with up to sixteen channels in sequential mode.
• Lambda scanning mode enables accurate spectral unmixing of complex overlapping fluorescent signals.
• 730 nm or 785 nm lasers and an NIR-sensitive 1- or 2-channel GaAs detectors enable up to 6 channels for multiplexed imaging from violet to NIR (400 nm–890 nm).
• TruFocus Red enables stable and gentle NIR time-lapse imaging.
• X Line objectives offer the widest range of chromatic correction, from 400 nm – 1000 nm, for exceptional color accuracy during multicolor fluorescence imaging

• Use a low-magnification 1.25X or 2X objective to quickly capture a large field of view (FOV) map of whole specimens
• Identify regions of interest on the Overlay Map, then switch to a higher magnification objective for high-resolution confocal imaging down to 120 nm with Olympus Super Resolution technology (FV-OSR)
• Finalize your acquisition and get publication-ready microscope images with TruSight image processing

• Its hybrid scan unit uses a galvanometer scanner for precision scanning as well as a resonant scanner, ideal for high-speed imaging of live physiological events
• Capture video-rate images with a large FOV using the resonant scanner, featuring speeds from 30 frames per second (fps) at FN 18 all the way up to 438 fps using clip scanning
• Use the resonant scanner to observe fast phenomena, such as a beating heart, blood flow, or calcium ion (Ca2+) dynamics inside cells
• Switch between the galvanometer scanner and resonant scanner with the click of a button

• Olympus’ TruFocus unit helps maintain focus during live cell imaging despite changes in temperature or added reagents
• Its microscope’s high-sensitivity detector requires significantly less laser power while the resonant scanner reduces laser illumination time, lowering phototoxicity for more physiologically accurate confocal imaging data

• Refractive index match delivers an ideal focal volume, resulting in perfect volume reconstruction and enabling high-resolution confocal imaging of large living organisms
• Long working distances enable detailed microscopic imaging at depth
• See data unfold in real-time and easily observe structures with 3D reconstruction software

Its microscope captures images at low magnification, and then the Macro-to-Micro software module can automatically locate your objects of interest in the vessel or well and capture them at high magnification. This automated process dramatically reduces the time you spend on microscope operation.